Single-molecule techniques

This book takes researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. The manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores.